Wales are keen to develop more competition for places and that led to Sean Lynn looking at the next generation in his 33-strong squad.
Five uncapped players are in the matchday squad - Amy Williams and Kelsie Webster start and Stella Orrin, Lucy Isaac and Lily Hawkins are on the bench.
"I don't want to be coming into pre-season where an individual has trained really well but we've got no evidence from a game, so that's what the squad is selected on," said Lynn.
"Certain individuals have put their hands up, and that's why we're going to give their reward."
Teenage scrum-half Hawkins only made her Gwalia Lightning debut in February.
'About the next generation'published at 16:49 BST 27 June
16:49 BST 27 June
Barbarians women v Wales women (17:00 BST)
Image source, Huw Evans Picture Agency
Wales women head coach Sean Lynn speaking on S4C: "The bits that we've really been working on these last few weeks is what we are going to be doing after half-time, keeping the ball is a big one and scoring more tries.
"It's really about this next generation of players, giving us some evidence of these individuals. The message I've given them is go out there and express why you should be playing for this Welsh side.
"They [the Barbarians] are going to be physical, they are going to throw the ball about, so we have just got to make sure that we can control the offload game as well."
Wales' PWR finalistspublished at 16:42 BST 27 June
16:42 BST 27 June
Barbarians women v Wales women (17:00 BST)
Image source, Huw Evans Picture Agency
The Premiership Women's Rugby final takes place just over the road at Twickenham Stoop tomorrow.
A raft of Wales players suffered disappointment when Gloucester-Hartpury lost to Ealing Trailfinders but there is still plenty of interest for Wales boss Sean Lynn.
Tight-head Donna Rose starts for Saracens with lock Georgia Evans on the bench, while Wales centre Carys Cox starts in midfield alongside England captain Meg Jones from Cardiff.
Wales' women take on the Baa-Baas and then have a short break before the WXV Global Series.
Sean Lynn also has a large contingent of players who are rested or unavailable after Premiership Women's Rugby action.
That has afforded the head coach the chance to have a look to the future, with the incentive of earning spots in the squad to face South Africa and USA in Cardiff in September - which is followed by two October Tests in Japan.
Five players are set for their international debuts - Amy Williams and Kelsie Webster start in the backs with Stella Orrin, Lucy Isaac and Lily Hawkins on the bench.
Wales are also lifted by the return of influential back-row forward Alex Callender, who captains the side after recovering from the ankle injury which meant she missed the Six Nations.
Kayleigh Powell gets the chance to put the pressure on Lleucu George after being moved from full-back to fly-half.
Wales women: N Metcalfe; Webster, Keight, De Vera, Williams; Powell, Lockwood; Davies, Reardon, Tuipulotu, B Metcalfe, Aiono, A Joyce, Callender (capt), King.
Replacements: Jones, Baverstock, Orrin, A Pyrs, Isaac, Hawkins, Bevan, Bell.
'That first half was a shock'published at 16:28 BST 27 June
16:28 BST 27 June
FT: Barbarians 31-33 Wales
Image source, Huw Evans Picture Agency
Dan Edwards speaking on S4C: "Yeah it was really warm, that first half was a shock to the system after not playing for six weeks. It was tough.
"It was a hard game to play in but it was fun out there today and I was playing with a smile on my face, which is the main thing.
"We had to focus on ourselves and what we can do, that was the message through the week. There was good stuff out there today and things we need to work on looking ahead to Fiji next week."
'Today has been special' - Northpublished at 16:24 BST 27 June
16:24 BST 27 June
FT: Barbarians 31-33 Wales
Image source, PA Media
George North speaking on S4C: "To be honest I was hoping for three tries! I thought my last game had happened. I had a phone call from Robbie [Deans, Barbarians coach] asking how my body was, he asked me to play and this week has been special.
"I wasn't ready to be honest when I came on. I was thinking just keep hold of the ball, take a deep breath and go.
"Today has been special, especially after a tough season. To get the chance to play today with my sons in the crowd who were so excited to see their dad play again.
"This game has given me everything. It was a childhood dream, I came from a place where you don't get many chances. From the very first game it has been a privilege every time I put the shirt on."
'Some things to work on'published at 16:15 BST 27 June
16:15 BST 27 June
FT: Barbarians 31-33 Wales
Image source, Huw Evans Picture Agency
Aaron Wainwright speaking on S4C: 'It was tough and very hot and humid. That was perfect for the conditions for what we will be coming up against in the summer.
"There were some good parts and some things to work on, so that's probably everything we wanted to get out of it.
"We spoke in camp about concentrating on ourselves and making sure we have our processes in place during the game. You never know what the Barbarians are going to throw at you, they'll try something new with an expansive style of play.
"We showed in parts what we can do when we took control of the game, but on the flip side when it got messy there were some areas that we need to tidy up."