Droylsden striker David McNiven scored three minutes into added time to end Worcester City's three-game unbeaten Blue Square Bet North run.
Defender Mark Clyde put City ahead at the Butchers Arms with a goal after only seven minutes.
But Clyde had a second effort ruled out for pushing before Colin Cryan levelled for the home side on 54 minutes.
McNiven's late winner was harsh on a City side that did more than enough to have taken something from the game.
City manager Carl Heeley told BBC Hereford and Worcester:
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"Again I am talking about a poor official who has booked their number 10, for what was a clear right hook on the back of Ryan Clarke's head.
"The last time I looked at the rules of playing football that is a sending-off and, immediately after the incident, they substituted him.
"We're nearly halfway through the season and this appalling standard of officials is continuing."
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